Your colleague in the research laboratory discovers a new Type 3 secretion system (T3SS) effector X that interferes with recruitment of a host protein Rab32 (that normally coats Salmonella-containing vacuoles, SCV). They suspect that Rab32 is proteolytically cleaved by effector X but find out that this is not the case. What experiment do you think your colleague might have done to arrive at this conclusion? So now you now suspect that effector X prevents localization of Rab32 to SCV by a different mechanism. Hypothesize a mechanism of how effector X interferes with Rab32 recruitment and suggest how you would experimentally test this.

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